منابع مشابه
Transient transfection of ecotropic retrovirus receptor permits stable gene transfer into non-rodent cells with murine retroviral vectors.
Retroviral vectors are useful for efficient, stable and single copy transfer of foreign genes into animal cells. However, the low risk murine ecotropic vectors can only be used with rodent cells due to their restricted host range. When using cells from other species, in particular human, amphotropic retroviral vectors and the corresponding packaging cell lines are required. Given the wide host ...
متن کاملRetroviral Replication
The retroviruses (the taxonomic family Retroviridae) comprise isolates of vertebrates, but elements with hallmarks of retroviruses are also found elsewhere, e.g. in insects. Retroviruses exemplify a variety of specialized interactions with their hosts, including induction of many kinds of diseases under laboratory conditions as well as in domestic and wild animals. In contrast to this variation...
متن کاملRetroviral Genotoxicity
Gene therapies have enormous potential to cure human disease. In recent years, hematopoiet‐ ic stem cell (HSC) gene therapy has advanced tremendously, due in part to years of intense re‐ search to develop effective vectors and efficient ex vivo transduction protocols. In early clinical trials, inefficient gene transfer resulted in either a lack of therapeutic benefit or shortlived therapeutic b...
متن کاملPolysaccharide gene transfection agents.
Gene delivery is a promising technique that involves in vitro or in vivo introduction of exogenous genes into cells for experimental and therapeutic purposes. Successful gene delivery depends on the development of effective and safe delivery vectors. Two main delivery systems, viral and non-viral gene carriers, are currently deployed for gene therapy. While most current gene therapy clinical tr...
متن کاملTransfection in Streptomyces virginiae
Until now, transformation with linear DNA has not been demonstrated in Streptomycetes, although it has been established in a thermophilic Actinomyces species, Thermoactinomyces vulgaris (Hopwood & Wright, 1972). The main tool used in Streptomycete genetics is conjugation (Hopwood, 1967). In the wake of a genetic map for Streptomyces coelicolor, maps for other strains were constructed by means o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2012
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113901